Citation
To cite this calculator, please reference the following publication and include a link to this page:
- Fisher-Wellman, K.H., Davidson, M.T., Narowski, T.M., Lin, C.-T., Koves, T.R., and Muoio, D.M. (2018). Mitochondrial Diagnostics: A Multiplexed Assay Platform for Comprehensive Assessment of Mitochondrial Energy Fluxes. Cell Reports 24, 3593-3606.e10.
- PMID: 30257218
References
Teague, Golding, and Dobson were not involved in the generation of this site. However, their manuscript, constants, and equations formed the bedrock of this calculator.
- Teague, W. E., Golding, E. M. & Dobson, G. P. Adjustment of K’ for the creatine kinase,
adenylate kinase and ATP hydrolysis equilibria to varying temperature and ionic strength.
Journal of Experimental Biology 199, 509–512 (1996).
Additional References
- Golding, E. M., Teague, W. E. & Dobson, G. P. Adjustment of K’ to varying pH and
pMg for the creatine kinase, adenylate kinase and ATP hydrolysis equilibria permitting
quantitative bioenergetic assessment. Journal of Experimental Biology 198, 1775–1782 (1995).
- Alberty, R. A. Calculation of transformed thermodynamic properties of biochemical
reactants at specified pH and pMg. Biophysical Chemistry 43, 239–254 (1992).
- Clarke, E. C. W. & Glew, D. N. Evaluation of Debye–Hückel limiting slopes for water
between 0 and 150°C. J. Chem. Soc., Faraday Trans. 1 76, 1911–1916 (1980).
Links
- Source Code for this site
- An html rendition of the Jupyter notebook which explains the process of adjusting equilibrium constants over the creatine kinase clamp.
- A functional copy of the Jupyter notebook